强光照和黑暗条件下刺参体壁的基因表达差异     

张立斌  冯其明  孙丽娜  房燕  徐冬雪  张涛  杨红生 《海洋学报(英文版)》2018,37(5):54-66

参对光照变化非常敏感，研究刺参对光照的分子响应非常重要。本研究应用RNA测序获取了刺参暴露于强光（“强光”）、正常光照（“对照”）和完全黑暗（“黑暗”）环境下体壁的基因表达谱情况，通过“对照”与“黑暗”，“对照”与“强光”和“黑暗”与“强光”的比较，在|log₂ ratio|≥1和FDR≤0.001的标准下分别发现了1161、113和1705个差异表达基因（DEGs）。基因本体分析表明，“cellular process”和“binding”在“生物过程”和“分子功能”类别中的DEGs富集最多，而“cell”和“cell part”在“细胞组分”类别中的DEGs富集最多。将DEGs与Kyoto Encyclopedia基因和基因组数据库上的于214、41和229条通路进行比对，发现了51、2和57条通路分别显著富集。本研究发现的光特异性DEGs可作为深入研究刺参对光照变化的生化适应机制的重要目标基因。  相似文献   

6-磷酸山梨醇脱氢酶(gutD)基因克隆、测序和表达     

陈启伟  刘广发  邱凤英 《台湾海峡》2004,23(3):314-317

本研究根据6-磷酸山梨醇脱氢酶基因(gutD)两端序列设计引物，以假单胞杆菌(Pseudonmonas XM)的总DNA为模板通过PCR的方法克隆gutD基因并进行序列分析，将克隆得到的gutD基因与原核表达载体pBV220连接并转化大肠杆菌(Escherichia coli)JM101后检测其蛋白表达及菌株生长耐盐性。  相似文献   

Characterization of zebrafish metallothionein gene promoter in a zebrafish caudal fin cell-line,SJD. 1     

Yan CH  Chan KM 《Marine environmental research》2002,54(3-5):335-339

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A cDNA microarray technique applied for analysis of global gene expression profiles in tributyltin-exposed ascidians   总被引：2，自引：0，他引：2  

Azumi K  Fujie M  Usami T  Miki Y  Satoh N 《Marine environmental research》2004,58(2-5):543-546

To analyze global gene expressions, we constructed a cDNA microarray from a basal chordate, the ascidian Ciona intestinalis. Ciona is a cosmopolitan species and a genomic analysis of Ciona revealed that ascidians had approximately 15,500 protein-coding genes. Our "Ciona intestinalis cDNA chip version 1 (Ci cDNA chip ver. 1)" has arrayed 13,400 unique Ciona cDNAs. To establish a detection system for gene expression profiles in wild ascidians using a cDNA microarray, we analyzed gene expressions in the whole body of Ciona adults after exposure to 100 nM tributyltin (TBT) for 24 h. In our preliminary array data using Ci cDNA chip ver. 1, we found more than 200 genes that showed strong differential expressions. These genes encoded proteins that were concerned with stress response, detoxification, oxidoreduction reaction, biosynthesis, and catabolism. This, the first large cDNA microarray of this animal, should facilitate analyses of global gene expressions following exposure to TBT.  相似文献   

沉降监测多项式回归分析与神经网络预测     

田丰  文鸿雁  张静 《海洋测绘》2007,27(4):23-27

使用多项式和切比雪夫(Tchebyshev)多项式分别对沉降监测数据进行回归分析以预测未来沉降值,其中切比雪夫多项式的外推效果较好;应用前向BP神经网络对两种不同的单因子输入模式进行非线性函数逼近,并进行了不同采样步长的比较,实例表明将时间点作为网络的输入对沉降进行预测效果较好。  相似文献   

转兔防御素基因小球藻培养的营养模式研究     

韩兴梅  李元广  魏晓东  李兴武  孙勇如  王义琴 《海洋科学》2006,30(7):48-52

在250mL摇瓶中研究了光自养、异养、混合营养3种营养模式对转兔防御素基因(NP-1)小球藻(Chlorella)的生长、NP-1表达量、总蛋白质含量、pH、葡萄糖消耗以及光衰减等几个方面的影响。结果表明,异养培养为转NP-1基因小球藻的最适营养模式。在此基础上,采用5,15,30L生物反应器补料分批异养培养转NP-1基因小球藻,细胞质量浓度分别达到3.67,4.87,6.39g/L。  相似文献   

简化模糊H-ARTMAP神经网络在高光谱遥感分类中的应用     

张伟  杜培军  尹作霞 《测绘科学》2008,33(5)

人工神经网络作为一种不需估计类别分布参数的遥感影像分类方法,能够克服分类中的不确定性,提高分类精度。模糊ARTMAP人工神经网络具有稳定、泛化性能好、支持增量式学习等特点,通过对简化模糊AR-TMAP神经网络和H-ARTMAP神经网络的分析和集成,构造了一种用于高光谱遥感影像分类的简化模糊H-ART-MAP网络。实验证明该方法在分类效率、运算时间和分类精度等方面都优于最大似然分类、BP神经网络、最小距离分类、光谱角制图模型等分类方法。  相似文献   

福建平潭虎潮山滑坡成因机理分析与评价   总被引：3，自引：0，他引：3  

秦刚  姚环  林燕滨 《中国地质灾害与防治学报》2006,17(3):10-13

虎潮山滑坡位于平宏公路12k 704~12k 884段北侧山坡体上,是平潭海岛通往陆地的唯一公路通道。论文通过定性的工程地质分析和定量计算评价了虎潮山坡体稳定性的变化趋势,提出改建公路超挖深切坡脚和持续长时间强降雨导致的孔隙水压力作用是边坡发生滑动破坏的主要因素。  相似文献   

Recent developments in seismically isolated buildings in Japan     

Shin Okamoto  Nagahide Kani  Masahiko Higashino  Norihide Koshika  Masayuki Kimizuka  Mitsumasa Midorikawa  Masanori Iiba 《地震工程与工程振动(英文版)》2002,1(2):213-225

The Building Standard Law of Japan and related Enforcement Order and Notifications have been substantially revised since the year 2000 to introduce a performance-based regulatory and deregulation system for building control systems. Up to then, time-history analyses were mandatory for isolated buildings and had to be specially approved by the Minster of the Ministry of Construction (MOC). Simplified design procedures based on the equivalent linear method for seismically isolated buildings have been issued as “Notification 2009 — Structural calculation procedure for buildings with seismic isolation” from MOC, and are now integrated into the Ministry of Land, Infrastructure, and Transportation (MLIT). Along with Notification 2009, “Notification 1446 of year 2000 — Standard for specifications and test methods for seismic isolation devices” was also issued. Buildings with heights equal to or less than 60m and that are designed according to these Notifications, including base isolated buildings, only need approval from local building officials, and no longer require the special approval of the Minister of MLIT. This paper summarizes: 1) some statistics related to buildings with seismic isolation completed up to the end of 2001; 2) simplified design procedures required by Notification 2009 of year 2000; and 3) performance of seismic isolation devices required by Notification 1446 of year 2000.  相似文献   

3-D rheologic model of earthquake preparation (I)—Displacement field     

宋治平  尹祥础  梅世蓉 《地震学报(英文版)》2003,(6)

Introduction The rock medium shows mainly elastic character under low-temperature, low-pressure and external force for short duration, yet it shows rheologic property under high-temperature, high-pressure and long-time external force. It is reasonable that the rapid earthquake generating process lasted for tens seconds is analyzed by the elastic theory. As for the earthquake generating process for hundreds or thousand years, nevertheless, the rheologic property of rock must be taken into accou…  相似文献